Figure 5

Effect of orally fed BLS-mix on intestinal lymphocytes. A Representative dot plots showed the gating strategy for gut T-cell subpopulations. Flow cytometry analysis of the percentage of B CD4⁺CD8⁻, C CD4⁻CD8⁺, D CD4⁺CD8⁺, E CD4⁻CD8⁻ cells among intestinal CD3⁺ T cells. Peyer’s patch lymphocytes (PPLs), intraepithelial lymphocytes (IELs), and lamina propria lymphocytes (LPLs) were collected from jejunal and ileal tissues from the indicated pigs 1 week after F4⁺ ETEC/VTEC/EPEC challenge. Data are presented as the mean ± SEM for each tissue (n = 8 per group). *P < 0.05; **P < 0.01; ***P < 0.001.