Figure 5

IBV RNA in tracheal secretions, plasma and mononuclear cells. Samples were collected from IBV B1648 and M41, and PBS (mock) inoculated chickens at 0, 2, 4, 6, 8, 10 and 12 dpi. Viral RNA was measured by RT-qPCR. Solid and dashed lines represent mean viral RNA copies per strain and per day. Mock is represented by the dash-dotted line. The quantification limit of RT-qPCR was 3.4 log₁₀ copies/mL (dotted line). The detection limit of RT-qPCR was 2.4 log₁₀ copies/mL (bold dotted line).