Figure 3From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells Expression kinetics of TLRs mRNA. A–C Relative expression of TLRs mRNA, accounting for the effects of culture conditions on gene transcription in WNV- and mock-inoculated equine PBMCs. To compare the normalised expression of TLRs genes from PBMCs harvested at each time point to their respective expression levels before either WNV- or mock- inoculation, the ∆∆Ct values were calculated by subtracting ∆Ct of genes in fresh-isolated PBMCs from the ∆Ct of genes in WNV- or mock- inoculated PBMCs at each time-point (for WNV-stimulated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{WNV}} = \Delta {\text{Ct}}_{\text{WNV}} - \Delta {\text{Ct}}_{\text{fresh}}\); and for mock-inoculated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{mock}} = \Delta {\text{Ct}}_{\text{mock}} - \Delta {\text{Ct}}_{\text{fresh}}\)). *P < 0.05.Back to article page