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Figure 3 | Veterinary Research

Figure 3

From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Figure 3

Expression kinetics of TLRs mRNA. A–C Relative expression of TLRs mRNA, accounting for the effects of culture conditions on gene transcription in WNV- and mock-inoculated equine PBMCs. To compare the normalised expression of TLRs genes from PBMCs harvested at each time point to their respective expression levels before either WNV- or mock- inoculation, the ∆∆Ct values were calculated by subtracting ∆Ct of genes in fresh-isolated PBMCs from the ∆Ct of genes in WNV- or mock- inoculated PBMCs at each time-point (for WNV-stimulated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{WNV}} = \Delta {\text{Ct}}_{\text{WNV}} - \Delta {\text{Ct}}_{\text{fresh}}\); and for mock-inoculated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{mock}} = \Delta {\text{Ct}}_{\text{mock}} - \Delta {\text{Ct}}_{\text{fresh}}\)). *P < 0.05.

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