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Figure 3 | Veterinary Research

Figure 3

From: Serum-derived exosomes from non-viremic animals previously exposed to the porcine respiratory and reproductive virus contain antigenic viral proteins

Figure 3

ELISA assay for evaluation of NV and CN porcine sera immune recognition over inactivated viral particle PRRSV vaccine (Porcilis PRRS, Intervet) and exosomes derived from NV porcine sera of different origin. Analyses of naïve (CN) and non-viremic (NV) sera against exosomes derived from sample 201502-1PS (A), 201502-2PS (B) and 201502-3PS (C). Optical density (OD) was measured at 450 nm and it is represented in the “Y” axis. “X” axis shows the dilution factor for sera samples (1/50 to 1/400). For each animal, exosomes were isolated and captured using anti-CD63 antibody and tested against both sera (**p < 0.01; ***p < 0.0001).

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