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Figure 1 | Veterinary Research

Figure 1

From: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines

Figure 1

Sequence comparison, phylogenetic tree of amino acid and western blot analysis of ChIL-26. A Chicken IL-10 (Accession # NP 001004414) and ChIL-26 amino acid sequences were aligned and secondary helices of IL-10 and IL-26 were shown in vertical and horizontal lined box, respectively. B Phylogenetic tree was constructed by Phylodendron program using amino acid sequences aligned with BioEdit and Mega4 and (C) Western blot analysis of ChIL-26 recombinant protein. E. coli BL21(DE3) transformed with the pET32a(+)-ChIL-26 plasmid was induced with 1.0 mM IPTG, purified with a HisPur Cobalt resin and analyzed by Western blotting using anti-His (C-Term)-HRP Antibody.

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Veterinary Research

ISSN: 1297-9716