From: Epidemiological characteristics of infectious hematopoietic necrosis virus (IHNV): a review
Species | Tissue | Source of virus contamination | Test conditions (°C) | Starting titre | Titre reduction (log10)/virus detection | Reference |
---|---|---|---|---|---|---|
Rainbow trout | Homogenate of kidney, spleen and heart | 7–9 cm fish from a farm with a recent history of IHN | 4 | 104.8 TCID50 mL−1 | Titre reduced by about 1–2 log per day. Virus survived 3 (but not 4) days | [134] |
25Â | No evidence of virus after 1Â day | |||||
Rainbow trout | Homogenate of muscle and skin from fingerlings (fish minus heads tails and viscera) | Seeded with IHNV | 4 | 105.7 TCID50 mL−1 | Titre reduced by 3 logs after 7 days | [140] |
10Â | Titre reduced by 4 logs after 5Â days | |||||
21Â | Titre reduced by 3 logs after 4Â days | |||||
Rainbow trout | Individual homogenates of brain, liver, kidney and spleen from 3 fish of approx 900 g | Seeded with IHNV | −20 | 103.6–103.9 pfu mL−1 | Virus survived for 5 months in brain, 3.5 months in liver and spleen and 4 weeks in kidney tissue | [150] |
4Â | Virus survived at least 5Â weeks in brain, 4Â weeks in liver, 3Â weeks in spleen and was detected after 3 but not 7Â days in kidney tissue | |||||
20Â | Virus survived at least 1Â week in brain, losing 1 log titre; survived 1Â day in liver or spleen. Virus detected intermittently over a 3Â day period in kidney, but not after 1Â week | |||||
Sockeye salmon | Fry | Seeded with IHNV | −20 | 103.7 pfu mL−1 | Virus survived 4 weeks | [150] |
4Â | Detected after 3 but not 4Â weeks | |||||
20Â | Detected after 4Â h but not 24Â h | |||||
Sockeye salmon | Homogenate of gut, pyloric ceca, kidney, spleen and liver | Seeded with IHNV | 38 | >107 pfu mL−1 | c. 5 logs in <50 min | [141] |
32Â | c. 4 logs in 2.5Â h | |||||
28Â | c. 4 logs in 2.5Â h | |||||
8Â | No significant loss after c 3Â h |