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Table 2 Survival of infectious hematopoietic necrosis virus in fish tissues

From: Epidemiological characteristics of infectious hematopoietic necrosis virus (IHNV): a review

Species

Tissue

Source of virus contamination

Test conditions (°C)

Starting titre

Titre reduction (log10)/virus detection

Reference

Rainbow trout

Homogenate of kidney, spleen and heart

7–9 cm fish from a farm with a recent history of IHN

4

104.8 TCID50 mL−1

Titre reduced by about 1–2 log per day. Virus survived 3 (but not 4) days

[134]

25 

No evidence of virus after 1 day

Rainbow trout

Homogenate of muscle and skin from fingerlings (fish minus heads tails and viscera)

Seeded with IHNV

4 

105.7 TCID50 mL−1

Titre reduced by 3 logs after 7 days

[140]

10 

Titre reduced by 4 logs after 5 days

21 

Titre reduced by 3 logs after 4 days

Rainbow trout

Individual homogenates of brain, liver, kidney and spleen from 3 fish of approx 900 g

Seeded with IHNV

−20 

103.6–103.9 pfu mL−1

Virus survived for 5 months in brain, 3.5 months in liver and spleen and 4 weeks in kidney tissue

[150]

4 

Virus survived at least 5 weeks in brain, 4 weeks in liver, 3 weeks in spleen and was detected after 3 but not 7 days in kidney tissue

20 

Virus survived at least 1 week in brain, losing 1 log titre; survived 1 day in liver or spleen. Virus detected intermittently over a 3 day period in kidney, but not after 1 week

Sockeye salmon

Fry

Seeded with IHNV

−20 

103.7 pfu mL−1

Virus survived 4 weeks

[150]

4 

Detected after 3 but not 4 weeks

20 

Detected after 4 h but not 24 h

Sockeye salmon

Homogenate of gut, pyloric ceca, kidney, spleen and liver

Seeded with IHNV

38 

>107 pfu mL−1

c. 5 logs in <50 min

[141]

32 

c. 4 logs in 2.5 h

28 

c. 4 logs in 2.5 h

8 

No significant loss after c 3 h