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Table 1 Experimental design

From: Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes

Group name

No. of animals

Route of inoculation

Inoculum

1st day of challenge (0 dpi)

2nd day of challenge (1 dpi)

Control

2

Endotracheal

5 mL PBS

5 mL PBS

2

Intranasal

2

Aerosol

ET

8

Endotracheal

5 mL M. hyopneumoniae fresh culture

5 mL M. hyopneumoniae fresh culture

IN

8

Intranasal

AE

8

Aerosol

  1. The inoculation took place in 2 consecutive days (0 and 1 dpi). All pigs were euthanized at 4 weeks after the first inoculation (28 dpi).