Figure 6

Quantitiative PCR of cytokine mRNA. Cytokine mRNA expression as relative copy number. A The pro-inflammatory cytokine IL1α did not differ significantly between any of the samples. Both IL1β and IL6 were significantly higher in spleen† compared skin but not to sarcoid, skin and sarcoid did not differ significantly from each other. B No significant differences in IFNα or IFNβ levels among the samples were detected. IFNγ was significantly higher in spleen†† compared to either sarcoid or skin, which were not significantly different from each other. C There were no significant differences in IL12-p40 or IL12p35 mRNA between the different tissues while EBI3 (IL27βsubunit) was significantly elevated in the spleen†† compared to both skin and sarcoid which were not significantly different from each other. D TGFβ and FOXP3 were present in high copy numbers in all tissues there was no difference between sarcoid and spleen but both sarcoid* and spleen† were significantly higher compared to skin. IL10 was significantly higher in spleen†† compared to skin and sarcoid which in turn did not differ significantly from each other. E T-cell cytokines IL2 and IL4 were significantly higher in spleen†† compared to skin or sarcoid which were not significantly different from each other. Conversely IL17 was significantly lower in spleen** than either skin or sarcoid.