FOXP3 expression in sarcoid E2 positive tumour cell nuclei. A Sarcoid tissue stained with rabbit anti-FOXP3 texas-red. B Sarcoid tissue stained with mouse monoclonal anti-BPV E2 FITC. C dual-stained image with DAPI nuclear counterstain. The majority of nuclei in this image are dual positive tumour cells but some FOXP3 only T-cells are present (red arrow). D Higher power image (×1000) of nuclei stained with rabbit anti-FOXP3 texas red. E The same nuclei stained with mouse anti-BPV E2 FITC. F Higher power image (1000X oil immersion) of dual FOXP3 texas-red and BPV E2 FITC dual-positive sarcoid nuclei, DAPI counterstain.