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Table 1 Chronological description of experimental procedure

From: Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees

1 day-old individuals 3 day-old 4 day-old 13 day-old
Cage rearing
Diet: bee bread, sucrose solution (66%) and water ad libitum
Individual inoculation with or without N. ceranae spores in sucrose solution Beginning of the administration of acaricide or sucrose only solution for controls
Daily replacement of solutions until day 13 post emergence
Sampling bees for individual quantification of spores in midgut and individual gene expression analysis