Table 1 Chronological description of experimental procedure
1 day-old individuals | 3 day-old | 4 day-old | 13 day-old |
|---|---|---|---|
Cage rearing Diet: bee bread, sucrose solution (66%) and water ad libitum | Individual inoculation with or without N. ceranae spores in sucrose solution | Beginning of the administration of acaricide or sucrose only solution for controls Daily replacement of solutions until day 13 post emergence | Sampling bees for individual quantification of spores in midgut and individual gene expression analysis |