Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

Table 1 Immunoproteins identified by MALDI-TOF/TOF MS

Spot no.	Protein identified ^a	BLASTX similarity matched protein/species/identity score	Theoretical MW/pI ^b	Experimental MW/pI	MASCOT score ^c	No. of peptides matched ^d	Coverage (%)^e
BF1	gi\|338846659	DNA polymerase III delta subunit	39 994/5.80	22 000/5.70	252	19	55
BF2	gi\|195977415	elongation factor G	76 540/4.83	76 000/4.80	232	20	40
BF3	gi\|195978009	pyruvate kinase	54 638/5.09	55 000/5.10	374	36	62
BF4	gi\|338847723	Transketolase	71 341/5.07	71 000/5.09	272	11	21
BF5	gi\|338846410	chaperone protein DnaK (heat shock protein 70)	65 045/4.64	60 000/4.60	269	22	36
BF6	gi\|225867742	60 kDa chaperonin GroEL	56 876/4.70	56 000/4.60	575	34	60
BF7	gi\|195978262	30S ribosomal protein S1	43 801/4.93	43 000/5.00	172	16	46
BF8	gi\|338848099	inosine-5′-monophosphate dehydrogenase	53 007/5.48	53 000/5.60	257	29	63
BF9	gi\|338846419	3-oxoacyl-(acyl-carrier-protein) synthase II	43 738/5.44	40 000/5.40	164	17	52
BF10	gi\|338847987	adenylosuccinate synthetase	47 501/5.47	47 000/5.60	155	18	49
BF11	gi\|225869251	phosphoglycerate kinase	42 184/4.96	42 000/4.90	120	14	38
BF12	gi\|225867788	glucose-6-phosphate isomerase	49 490/4.88	42 000/4.80	207	23	48
BF13	gi\|225869252	glyceraldehyde-3-phosphate dehydrogenase	37 025/5.57	40 000/5.00	243	24	58
BF14	gi\|338846346	pyridine nucleotide-disulfide oxidoreductase	47 715/5.30	47 000/5.30	191	21	56
BF15	gi\|338847824	oligopeptide ABC transporter periplasmic oligopeptide-binding protein OppA	72 742/5.56	72 000/5.60	244	25	49
BF16	gi\|338847405	Elongation factor-Tu	44 545/4.89	21 000/5.30	109	9	22
BF17	gi\|225867729	elongation factor Ts	37 263/4.86	30 000/4.40	193	20	59

^agi number in NCBI.
^bTheoretical MW and pI was calculated using compute pI/MW [38].
^cMASCOT score obtained for the peptide mass fingerprint (PMF). The significance threshold was 70.
^dNumber of peptides that match the predicted protein sequence.
^ePercentage of predicted protein sequence covered by matched peptides.

ISSN: 1297-9716