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Table 1 Immunoproteins identified by MALDI-TOF/TOF MS

From: Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

Spot no. Protein identified a BLASTX similarity matched protein/species/identity score Theoretical MW/pI b Experimental MW/pI MASCOT score c No. of peptides matched d Coverage (%)e
BF1 gi|338846659 DNA polymerase III delta subunit 39 994/5.80 22 000/5.70 252 19 55
BF2 gi|195977415 elongation factor G 76 540/4.83 76 000/4.80 232 20 40
BF3 gi|195978009 pyruvate kinase 54 638/5.09 55 000/5.10 374 36 62
BF4 gi|338847723 Transketolase 71 341/5.07 71 000/5.09 272 11 21
BF5 gi|338846410 chaperone protein DnaK (heat shock protein 70) 65 045/4.64 60 000/4.60 269 22 36
BF6 gi|225867742 60 kDa chaperonin GroEL 56 876/4.70 56 000/4.60 575 34 60
BF7 gi|195978262 30S ribosomal protein S1 43 801/4.93 43 000/5.00 172 16 46
BF8 gi|338848099 inosine-5′-monophosphate dehydrogenase 53 007/5.48 53 000/5.60 257 29 63
BF9 gi|338846419 3-oxoacyl-(acyl-carrier-protein) synthase II 43 738/5.44 40 000/5.40 164 17 52
BF10 gi|338847987 adenylosuccinate synthetase 47 501/5.47 47 000/5.60 155 18 49
BF11 gi|225869251 phosphoglycerate kinase 42 184/4.96 42 000/4.90 120 14 38
BF12 gi|225867788 glucose-6-phosphate isomerase 49 490/4.88 42 000/4.80 207 23 48
BF13 gi|225869252 glyceraldehyde-3-phosphate dehydrogenase 37 025/5.57 40 000/5.00 243 24 58
BF14 gi|338846346 pyridine nucleotide-disulfide oxidoreductase 47 715/5.30 47 000/5.30 191 21 56
BF15 gi|338847824 oligopeptide ABC transporter periplasmic oligopeptide-binding protein OppA 72 742/5.56 72 000/5.60 244 25 49
BF16 gi|338847405 Elongation factor-Tu 44 545/4.89 21 000/5.30 109 9 22
BF17 gi|225867729 elongation factor Ts 37 263/4.86 30 000/4.40 193 20 59
  1. agi number in NCBI.
  2. bTheoretical MW and pI was calculated using compute pI/MW [38].
  3. cMASCOT score obtained for the peptide mass fingerprint (PMF). The significance threshold was 70.
  4. dNumber of peptides that match the predicted protein sequence.
  5. ePercentage of predicted protein sequence covered by matched peptides.