Figure 4
From: BHV-1 induced oxidative stress contributes to mitochondrial dysfunction in MDBK cells
Effect of BHV-1 on ATP content during the infection of MDBK cells. MDBK cells of confluent in 6-well plates were infected with BHV-1 at MOI of 0.01 for indicated time length. The content of ATP and protein concentration was determined with ATP Assay Kit and Bradford Protein Assay Kit, respectively. The ATP content was normalized to the protein concentrations and expressed as a % of mock treated control cells. Data represent three independent experiments. Statistical analyses were performed using Student’s t test (Asterisk indicates P < 0.05 vs. control).