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Table 1 Determination of virus titers of a WSSV stock in P. vannamei by different inoculations.

From: Per os infectivity of white spot syndrome virus (WSSV) in white-legged shrimp (Litopenaeus vannamei) and role of peritrophic membrane

Experiment Inoculation route Dilution on homogenate of tissue No. of shrimp Mortality at different time points (hpi) Infection by IIF Virus titer
24 36 48 60 72 84 96 120 Total
1 Intramuscular 10−7 5   1 1 1   1    4/5 4/5 108.8SID50/g
10−8 5     1      1/5 1/5
10−9 5          0/5 0/5
Peroral 100 5    1 1      2/5 2/5 101.1SID50/g
10−1 5          0/5 0/5
10−2 5          0/5 0/5
10−3 5          0/5 0/5
Feed 0.5 5          0/5 0/5 100.6SID50/g
5 5   1        1/5 1/5
50 5          0/5 0/5
100 5   1        1/5 1/5
250 5   1 1       2/5 2/5
500 5      3     3/5 3/5
2 Intramuscular 10−6 5   1 3   1     5/5 5/5 108.8SID50/g
10−7 5    1 2 1     4/5 4/5
10−8 5     1      1/5 1/5
10−9 5          0/5 0/5
Peroral 100 5     2 1     3/5 3/5 101.5SID50/g
10−1 5          0/5 0/5
10−2 5          0/5 0/5
10−3 5          0/5 0/5
Feed 0.5 5          0/5 0/5 100.8SID50/g
5 5          0/5 0/5
50 5     1      1/5 1/5
100 5     2      2/5 2/5
250 5   2 1       3/5 3/5
  500 5   1   2 1     4/5 4/5
3 Intramuscular 10−6 5   2 3       5/5 5/5 108.7SID50/g
10−7 5    2 2      4/5 4/5
10−8 5          0/5 0/5
10−9 5          0/5 0/5
Peroral 100 5     1 1     2/5 2/5 101.1SID50/g
10−1 5          0/5 0/5
10−2 5          0/5 0/5
10−3 5          0/5 0/5
Feed 0.5 5          0/5 0/5 100.8SID50/g
5 5          0/5 0/5
50 5      1     1/5 1/5
100 5      2     2/5 2/5
250 5     1 1     2/5 2/5
500 5     2 1 1    4/5 4/5
  1. Intramuscular injection, peroral inoculation and feeding (feed).
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Veterinary Research

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