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Table 4 Seroconversion after vaccination of pigs with VSV*ΔG(N), VSV*ΔG(GP3ecto-Myc), VSV*ΔG(GP4-HA) and VSV*ΔG expressing IAV proteins.

From: Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs

Ab against PRRSV Ag (by ELISA) Vaccine ELISA Days after VRP vaccination Days after PRRSV challenge
0 14 28 63a 77 0 2 4 7 9 11 14 21 28
N VSV*ΔG(N) Positive/total pigs 0/2 n.d. 0/2 2/2 2/2 n.d. n.d. n.d. 2/2 n.d. n.d. 2/2 n.d. n.d.
S/P ratiob (positive pigs) 0 n.d. 0 0.7 0.9 n.d. n.d. n.d. 2.3 n.d. n.d. 3.0 n.d. n.d.
Range (positive pigs) 0 n.d. 0 0.6–0.7 0.8–1.0 n.d. n.d. n.d. 2.0–2.6 n.d. n.d. 2.9–3.0 n.d. n.d.
VSV*ΔG (control) Positive/total pigs 0/2 n.d. n.d. n.d. 0/2 0/2 0/2 0/2 0/2 0/2 0/2 1/2 2/2 2/2
S/P ratio (positive pigs) 0 n.d. n.d. n.d. 0 0 0 0 0 0 0 0.6 0.7 0.7
Range (positive pigs) 0 n.d. n.d. n.d. 0 0 0 0 0 0 0 0.6 0.6–0.7 0.7–0.8
GP3 VSV*ΔG(GP3ecto-Myc) Positive/total pigs 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. n.d. 0/2 n.d n.d. 2/2 2/2 n.d.
VSV*ΔG (control) Positive/total pigs 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. 0/2 0/2 n.d.
N VSV*ΔG(GP3ecto-Myc) Positive/total pigs 0/2 n.d. n.d. n.d. n.d. 0/2 0/2 0/2 0/2 0/2 0/2 1/2 1/2 2/2
S/P ratio (positive pigs) 0 n.d. n.d. n.d. n.d. 0 0 0 0 0 0 0.5 1.3 1.0
Range (positive pigs) 0 n.d. n.d. n.d. n.d. 0 0 0 0 0 0 0.5 1.3 0.5–1.6
GP4 VSV*ΔG(GP4-HA) Positive/total pigs 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. 2/2 2/2 n.d.
VSV*ΔG (control) Positive/total pigs 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. n.d. 0/2 n.d. n.d. 0/2 0/2 n.d.
N VSV*ΔG(GP4-HA) Positive/total pigs 0/2 n.d. n.d. n.d. n.d. 0/2 0/2 0/2 0/2 0/2 0/2 1(1)/2 2/2 2/2
S/P ratio (positive pigs) 0 n.d. n.d. n.d. n.d. 0 0 0 0 0 0 0.6 0.6 0.7
Range (positive pigs) 0 n.d. n.d. n.d. n.d. 0 0 0 0 0 0 0.6 0.6–0.7 0.7
HA VSV*ΔG(HABelz) Positive/total pigs 0/2 2/2 2/2 2/2 2/2 n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a.
NA VSV*ΔG(NABelz) Positive/total pigs 0/2 2/2 2/2 2/2 2/2 n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a.
NP VSV*ΔG(NPBelz) Positive/total pigs 0/2 2/2 2/2 2/2 2/2 n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a.
M2 VSV*ΔG(M2Belz) Positive/total pigs 0/2 2/2 2/2 2/2 2/2 n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a.
  1. n.d.: not done; n.a.: not applicable.
  2. aAntibodies against GP3 and GP4 were also analysed at day 56. The results were 0/2.
  3. bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean). IDEXX X3 kit S/P ratios of >0.4 are considered positive. The S/P ratios represent the mean of 2 pigs, with the absolute values indicated under “range”.