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Table 3 Seroconversion after vaccination of pigs with VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) followed by PRRSV challenge.

From: Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs

Ab against PRRSV Ag (by ELISA) Vaccine ELISA Days after PRRSV challenge infection
0 2 4 7 9 11 14 24
GP5 VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) Positive/total pigs 0/4 0/4 0/4 2/4 2/4 4/4 4/4 4/4
% Competitiona (positive pigs) 0 0 0 44 ± 0.6 48 ± 4 55 ± 10 89 ± 13 89 ± 11
Range (positive pigs) 0 0 0 43–44 45–51 45–65 72–102 77–100
VSV*ΔG (control) Positive/total pigs 0/3 0/3 0/3 0/3 0/3 0/3 0/3 1/3
% Competition (positive pigs) 0 0 0 0 0 0 0 42
Range (positive pigs) 0 0 0 0 0 0 0 42
GP3 VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) Positive/total pigs 0/4 0/4 0/4 3/4 4/4 4/4 4/4 4/4
VSV*ΔG (control) Positive/total pigs 0/3 0/3 0/3 0/3 0/3 0/3 0/3 2/3
GP4 VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) Positive/total pigs 0/4 0/4 0/4 0/4 0/4 0/4 1/4 2/4
VSV*ΔG (control) Positive/total pigs 0/3 0/3 0/3 0/3 0/3 0/3 0/3 1/3
N VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) Positive/total pigs 0/4 0/4 0/4 0/4 0/4 0/4 1/4 4/4
S/P ratiob (positive pigs) 0 0 0 0 0 0 0.5 1.4 ± 0.4
Range (positive pigs) 0 0 0 0 0 0 0.5 0.9–1.9
VSV*ΔG (control) Positive/total pigs 0/3 0/3 0/3 0/3 1/3 1/3 1(1)/3 3/3
S/P ratio (positive pigs) 0 0 0 0 0.5 0.7 0.8 1.4 ± 0.7
Range (positive pigs) 0 0 0 0 0.5 0.7 0.8 0.8–2.1
  1. a% Competition = (negative control mean [mean of optical absorbance] − sample mean)/(negative control mean − positive control mean) × 100. The mean values are indicated with the standard deviation.
  2. bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean). IDEXX X3 kit S/P ratios of >0.4 are considered positive. The mean values are indicated with the standard deviation.