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Table 2 Seroconversion after vaccination of pigs with VSVΔG(GP5/M) followed by PRRSV challenge.

From: Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs

Ab against PRRSV Ag (by ELISA) Vaccine ELISA Days after PRRSV challenge infection
0 2 4 6 8 10 13 21
GP5 VSVΔG(GP5/M) Positive/total pigs 0/5 0/5 0/5 2/5 2/5 4/5 5/5 5/5
% Competitiona (positive pigs) 0 0 0 50 ± 12 49 ± 29 82 ± 15 82 ± 20 69 ± 15
Range for positive pigs 0 0 0 41–59 31–66 64–101 59–106 49–91
VSV*ΔG (control) Positive/total pigs 0/5 0/5 0/5 0/5 0/5 0/5 0/5 2/5
% Competition (positive pigs) 0 0 0 0 0 0 0 62 ± 13
Range for positive pigs 0 0 0 0 0 0 0 53–72
N VSVΔG(GP5/M) Positive/total pigs 0/5 0/5 0/5 0/5 0/5 2/5 4/5 4/5
S/P ratiob (positive pigs) 0 0 0 0 0 0.9 ± 0.4 1.0 ± 0.5 1.2 ± 0.1
Range (positive pigs) 0 0 0 0 0 0.5–1.2 0.6–1.7 1.1–1.3
VSV*ΔG (control) Positive/total pigs 0/5 0/5 0/5 0/5 0/5 0(1)/5 4/5 3/5
S/P ratio (positive pigs) 0 0 0 0 0 0 0.7 ± 0.2 1.4 ± 0.8
Range (positive pigs) 0 0 0 0 0 0 0.4–0.9 0.4–1.9
  1. IDEXX X3 kit S/P ratios of >0.4 are considered positive. The mean values are indicated with the standard deviation.
  2. a% Competition = (negative control mean [mean of optical absorbance] − sample mean)/(negative control mean − positive control mean) × 100. The mean values are indicated with the standard deviation.
  3. bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean).