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Figure 1 | Veterinary Research

Figure 1

From: Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs

Figure 1

Characterization of VRP-mediated expression of GP5 and M. A Immunofluorescence analysis of MARC-145 cells 6 h after infection with VSV*ΔG(GP5-HA), VSV*ΔG(M-Flag), VSVΔG(GP5/M) or with the VSV*ΔG control VRP. In the top panels, expression of GP5 is detected using the anti-GP5 mAb 3AH9. In the bottom panels, expression of M is detected using the anti-M mAb 11E10C7. B Western blot analysis of lysates from non-infected cells (lane 1) or cells infected with VSV*ΔG (lane 2), VSVΔG(GP5/M) (lane 3), PRRSV Olot/91 (lane 4) or mock (lane 5). The proteins were separated by SDS-PAGE under reducing (+βME) conditions and blotted onto a nitrocellulose membrane. The GP5 and M proteins were detected with the anti-GP5 and anti-M mAbs and with a porcine anti-PRRSV serum that reacted also with N. α-tubulin served as loading control using the anti-α-tubulin mAb. Protein molecular weight (kDa) standards are indicated.

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