Compound
|
IC50 Nc
|
IC50 HFF
|
Reference
|
---|
Artemiside
|
346 (308–388)
|
>5000
|
This work
|
Artemisone
|
3 (1–13)
|
>5000
|
[16]
|
Buparvaquone
|
4.9 (4.0–5.9)
|
>5000
|
[30]
|
Mefloquine
|
941 (775–1141)
|
3643 (3062–4335)
|
This work
|
- Human foreskin fibroblasts (HFF) were grown to confluence in 96-well-plates, treated with the compounds at various concentrations or with DMSO as a solvent control, and after 3 days viability was measured using the Alamar blue assay. To determine the inhibition of N. caninum (Nc) tachyzoite proliferation (Nc), HFF monolayers were infected with Nc-beta-gal tachyzoites (103 per well). After 3 days, beta-galactosidase activity was determined. IC50 values (inhibitory concentration of 50% of the solvent control value) were calculated as described [16] and are given in nM (with 95% confidence intervals).