Compound | IC50 Nc | IC50 HFF | Reference |
|---|
Artemiside | 346 (308–388) | >5000 | This work |
Artemisone | 3 (1–13) | >5000 | [16] |
Buparvaquone | 4.9 (4.0–5.9) | >5000 | [30] |
Mefloquine | 941 (775–1141) | 3643 (3062–4335) | This work |
- Human foreskin fibroblasts (HFF) were grown to confluence in 96-well-plates, treated with the compounds at various concentrations or with DMSO as a solvent control, and after 3 days viability was measured using the Alamar blue assay. To determine the inhibition of N. caninum (Nc) tachyzoite proliferation (Nc), HFF monolayers were infected with Nc-beta-gal tachyzoites (103 per well). After 3 days, beta-galactosidase activity was determined. IC50 values (inhibitory concentration of 50% of the solvent control value) were calculated as described [16] and are given in nM (with 95% confidence intervals).
Veterinary Research