Cerebral parasite load in non-pregnant and pregnant mice treated with buparvaquone. BALB/c mice were infected with Nc-Spain7 tachyzoites and subsequently treated with buparvaquone (BPQ) in corn oil or corn oil only (Placebo) as detailed in Section “Materials and methods”. Non-pregnant mice were euthanized 3 weeks pi, dams were euthanized 30 days post partum (pp), thus 44 days pi. After euthanasia, brains were collected and the amount of tachyzoites was determined by quantitative PCR and are presented as box plots (**p < 0.001; Kruskal–Wallis-test). A non pregnant mice, B dams.