Modulating APN expression affects ETEC adherence.
A Adhesion of F4 E. coli strains to IPEC-J2 cells. Bacterial adherence to the original IPEC-J2 cell line was normalized to 100%. B In vitro inhibition assay. Adherence of F4+ ETEC to IPEC-J2 cells after pre-incubation with anti-F4 fimbriae monoclonal antiserum (1:100 dilution) or with anti-APN polyclonal antiserum (1:1, 1:10, 1:100 dilutions). The adhesion of the untreated samples was normalized 100%. The experiments were repeated three times and data are expressed as mean ± standard deviations (p < 0.05).