Table 1 Primer sequences for cloning Brucella abortus genes
Gene | Primer sequence (5′–3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
Outer membrane protein 28 | F: GATCGGATCCAACACTCGTGCTAGCAATTTT | 63 | 753 |
R: GATCAAGCTTTTACTTGATTTCAAAAACGAC | |||
Malate dehydrogenase | F: AATTCGGATCCATGGCACGCAACAAGATT | 63 | 963 |
R: AGGCGTCGACTTATTTCAGCGACGGAGC | |||
Elongation factor | F: AATTCGAATTCATGAGCATTTCCGCATCT | 63 | 918 |
R: AGGCCTGCAGTCAGCCCTTGGCGGCTGCGGC | |||
Arginase | F: AGCGCGGATCCATGCATTGTAAGATTCTG | 63 | 918 |
R: AGGCCTGCAGTCAATAGCTGATGGTCGG | |||
Metal-dependent hydrolase | F: AGCGCGGATCCATGCATTGTAAGATTCTG | 63 | 711 |
R: AGCGCTGCAGTTAAGCTTGGAAGCTGTG |