Genital plaque associated with
herpesvirus type 1 (PPHV-1) infection in a harbor porpoise. Genital mucosa of harbor porpoise #6 infected with PPHV-1, a gammaherpesvirus. Tissue sections are either stained with hematoxylin and eosin (HE) or by in situ hybridization (ISH) specific for RNA of PPHV-1. Left column shows normal genital mucosa and right column shows genital plaque infected with PPHV-1. The genital plaque is characterized by marked hyperplasia of the epithelial layer (top row). In the superficial part of this hyperplastic epithelium, many epithelial cells have nuclei with amphophilic intranuclear inclusion bodies, characteristic for herpesvirus infection (middle row). These nuclei express PPHV-2 RNA, visible as bright red staining (bottom row).