TLR2 ligands enhanced barrier function in IPEC-J2 cells. IPEC-J2 cells were treated with synthetic TLR2 ligands, Pam3CSK4 (PCSK), B. subtilis-derived PGN (PGN-BS) or B. subtilis-derived LTA (LTA-BS) at 0, 0.1, 1, or 10 μg/mL. A TEER was examined at 24 h using epithelial voltohm meter. Data are presented as mean ± SD (n = 4). *P < 0.05; **P < 0.01, determined by one-way ANOVA with Tukey’s posttest. B The monolayer of IPEC-J2 cells was lysed and protein extracts were analyzed for claudin-3, occludin, and ZO-1 by using Western blot assay. C Lysates were produced from membrane and cytosolic portion of the cells and the expression of claudin-3 and occludin at 24 h was examined by Western blot assay. β-actin was used as an internal control (n = 3). The representative figure from three similar results is shown. Cyt; cytosol, Mem; membrane.