Basal expression level of immune genes and its modulation after challenging with heat-killed
A mRNA copy numbers relative to similar RNA inputs of TNF, IL6 and CCL20 as measured from the different cell types, as indicated. cDNA copy numbers were titrated against external standards and normalized according to the amount of RNA input. Note the broken ordinate in the graph of TNF. B Visualization of the data from several genes using the EXPANDER software. Each line displays the relative copy number of the respective gene as indicated over the time [h] of the challenge (0, 1, 3, 24), normalized across all cell types to the average of 0 and variance 1. Data are taken from Additional file 3. Data are mean values (error bars, ±SEM) from two replica experiments, each assayed in duplicate.