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Table 3 Sequential titration of VHSV in the gills, brain, and kidney of fish sampled at pre-determined time points in experiment 2, after exposure by immersion to various VHSV isolates.

From: Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout

Days post exposure Group Individual no. TCID50/g organ
Gill Brain Heart Kidney
1 DK-3592B 1 103.5 0a 103.6 <101.3b
  2 0 0 0 0
3 <101.3 0 0 0
NO-2007-50-385 1 h 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
NO-2007-50-385 6 h 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
3 DK-3592B 1 0 0 0 <101.3
2 0 <101.3 104.2 105.3
3 103.9 <101.3 104.9 106.0
NO-2007-50-385 1 h 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
NO-2007-50-385 6 h 1 0 0 0 0
2 0 <101.3 103.6 107.2
3 0 104.7 105.9 107.1
5 DK-3592B 1 108.1 106.8 109.4 109.3
2 106.9 105.9 108.2 107.5
3 103.9 0 105.1 103.9
NO-2007-50-385 1 h 1 106.9 105.1 108.1 107.6
2 104.0 103.7 0 <101.3
3 0 0 0 <101.3
NO-2007-50-385 6 h 1 105.9 107.3 107.2 107.7
2 103.2 0 105.2 0
3 0 <101.3 0 0
7 DK-3592B 1 105.9 10 4.9 108.2 106.2
2 104.1 0 106.8 104.5
3 103.6 0 106.3 103.6
NO-2007-50-385 1 h 1 0 0 0 0
2 0 0 0 0
3 <101.3 0 0 104.1
NO-2007-50-385 6 h 1 <101.3 0 <101.3 0
2 104.9 10 6.0 107.0 106.5
3 0 0 0 0
13 DK-3592B 1 108.3 <101.3 108.2 104.0
2 105.7 104.7 107.6 106.0  
NO-2007-50-385 1 h 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
NO-2007-50-385 6 h 1 0 0 <101.3 0
2 104.5 <101.3 105.1 105.1
3 0 0 0 0
20 NO-2007-50-385 1 h 1 0 0 0 0
2 0 103.1 0 0
3 0 103.6 0 0
NO-2007-50-385 6h 1 0 0 0 0
2 0 0 0 0
  1. a0, Not detected
  2. b<101.3, Positive but under limit for titration (<101.3 TCID 50/g)