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Table 3 Sequential titration of VHSV in the gills, brain, and kidney of fish sampled at pre-determined time points in experiment 2, after exposure by immersion to various VHSV isolates.

From: Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout

Days post exposure

Group

Individual no.

TCID50/g organ

Gill

Brain

Heart

Kidney

1

DK-3592B

1

103.5

0a

103.6

<101.3b

 

2

0

0

0

0

3

<101.3

0

0

0

NO-2007-50-385 1 h

1

0

0

0

0

2

0

0

0

0

3

0

0

0

0

NO-2007-50-385 6 h

1

0

0

0

0

2

0

0

0

0

3

0

0

0

0

3

DK-3592B

1

0

0

0

<101.3

2

0

<101.3

104.2

105.3

3

103.9

<101.3

104.9

106.0

NO-2007-50-385 1 h

1

0

0

0

0

2

0

0

0

0

3

0

0

0

0

NO-2007-50-385 6 h

1

0

0

0

0

2

0

<101.3

103.6

107.2

3

0

104.7

105.9

107.1

5

DK-3592B

1

108.1

106.8

109.4

109.3

2

106.9

105.9

108.2

107.5

3

103.9

0

105.1

103.9

NO-2007-50-385 1 h

1

106.9

105.1

108.1

107.6

2

104.0

103.7

0

<101.3

3

0

0

0

<101.3

NO-2007-50-385 6 h

1

105.9

107.3

107.2

107.7

2

103.2

0

105.2

0

3

0

<101.3

0

0

7

DK-3592B

1

105.9

10 4.9

108.2

106.2

2

104.1

0

106.8

104.5

3

103.6

0

106.3

103.6

NO-2007-50-385 1 h

1

0

0

0

0

2

0

0

0

0

3

<101.3

0

0

104.1

NO-2007-50-385 6 h

1

<101.3

0

<101.3

0

2

104.9

10 6.0

107.0

106.5

3

0

0

0

0

13

DK-3592B

1

108.3

<101.3

108.2

104.0

2

105.7

104.7

107.6

106.0

 

NO-2007-50-385 1 h

1

0

0

0

0

2

0

0

0

0

3

0

0

0

0

NO-2007-50-385 6 h

1

0

0

<101.3

0

2

104.5

<101.3

105.1

105.1

3

0

0

0

0

20

NO-2007-50-385 1 h

1

0

0

0

0

2

0

103.1

0

0

3

0

103.6

0

0

NO-2007-50-385 6h

1

0

0

0

0

2

0

0

0

0

  1. a0, Not detected
  2. b<101.3, Positive but under limit for titration (<101.3 TCID 50/g)