Table 2 VHSV detection by reverse-transcription PCR and virus re-isolation in cell cultures inoculated with organs from fish that died during the experimental observation period, or those that survived and were harvested at 46 dpe for experiment 1, and 29 dpe for experiment 2.
From: Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout
Experiment | Group (genotype) | Treatment (infectious route/negative control) | Cumulative mortality (%) | Dead fish (no. of positive fish/no. of tested fish) | Surviving fish (no. of positive fish/no. of tested fish) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Kidney + spleen | Brain | Kidney + spleen | Brain | ||||||||
Virus re-isolation | RT-PCR | Virus re-isolation | RT-PCR | Virus re-isolation | RT-PCR | Virus re-isolation | RT-PCR | ||||
1 | DK-3592B (Ia) | IP | 65 | 12/13 | 11/13 | 13/13 | 9/13 | 0/7 | 0/7 | 0/7 | 0/7 |
Immersion 1 h | 75 | 15/15 | 14/15 | 15/15 | 15/15 | 0/5 | 0/5 | 0/5 | 0/5 | ||
NO-2007-50-385 (III) | IP | 20 | 3/4 | 3/4 | 4/4 | 4/4 | 0/16 | 0/16 | 0/16 | 0/16 | |
Immersion 1 h | 15 | 2/3 | 2/3 | 3/3 | 3/3 | 1/17 | 0/17 | 2/17 | 1/17 | ||
Immersion 6 h | 20 | 3/4 | 3/4 | 4/4 | 4/4 | 0/16 | 0/16 | 1/16 | 0/16 | ||
4p168 (III) | IP | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | |
Immersion 1 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | ||
Immersion 6 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | ||
Negative control | IP | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | |
Immersion 1 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | ||
2 | DK-3592B (Ia) | Immersion 1 h | 65 | 13/13 | 12/13 | 13/13 | 11/13 | 0/7 | 0/7 | 1/7 | 1/7 |
NO-2007-50-385 (III) | Immersion 1 h | 5 | 1/1 | 1/1 | 1/1 | 1/1 | 2/19 | 0/19 | 4/19 | 3/19 | |
Immersion 6 h | 35 | 7/7 | 7/7 | 7/7 | 7/7 | 3/13 | 0/13 | 3/13 | 3/13 | ||
4p168 (III) | Immersion 1 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | |
Immersion 6 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | ||
Negative control | Immersion 6 h | 0 | – | – | – | – | 0/20 | 0/20 | 0/20 | 0/20 | |