Table 1 Expression plasmids.
From: The non-structural protein μNS of piscine orthoreovirus (PRV) forms viral factory-like structures
Plasmid name | Primer | Sequence (5ʹ → 3ʹ) |
|---|---|---|
pcDNA3.1-μNS-N-FLAG | Forward | GCCGCTCGAGTCTAGAGCCACCATG GACTACAAAGACGATGACGACAAGATGGCTGAATCAATTACTTTTG |
Reverse | AAACGGGCCCTCTAGATCAGCCACGTAGCACATTATTCAC | |
pcDNA3.1-μNS-C-FLAG | Forward | GCCGCTCGAGTCTAGAGCCACCATGCGCAAGCTGGACTTGGTTGCA |
Reverse | AAACGGGCCCTCTAGATCACTTGTCGTCATCGTCTTTGTAGTCGCCACGTAGCACATTATTCACGCC | |
pcDNA3.1-σNS-N-MYC | Forward | GCCGCTCGAGTCTAGAGCCACCATG GAACAAAAACTCATCTCAGAAGAGGATCTGATGTCGAACTTTGATCTTGG |
Reverse | AAACGGGCCCTCTAGACTAACAAAACATGGCCATGA | |
pcDNA3.1-σNS-C-MYC | Forward | GCCGCTCGAGTCTAGAGCCACCATGTCGAACTTTGATCTTGG |
Reverse | AAACGGGCCCTCTAGACTACAGATCCTCTTCTGAGATGAGTTTTTGTTCACAAAACATGGCCATGATGC | |
pcDNA3.1-μ2-C-HA | Forward | GGCGGCCGCTCGAGTCTAGAATGCCTATCATAAACCTGCC |
Reverse | GTTTAAACGGGCCCTCTAGAAGCGTAATCTGGAACATCGTATGGGTACTCACCAGCTGTAGACCACC | |
pcDNA3.1- λ1-N-HA | Forward | CGCTCGAGTCTAGAGCCACCATG TACCCATACGATGTTCCAGATTACGCTATGGAGCGACTTAAGAGGAAAG |
Reverse | AAACGGGCCCTCTAGATTAGTTGAGTACAGGATGAG | |
pcDNA3.1-μNSΔ743-753 | Forward | GCCGCTCGAGTCTAGAGCCACCATG GACTACAAAGACGATGACGACAAGATGGCTGAATCAATTACTTTTG |
Reverse | AAACGGGCCCTCTAGATCACCAGTCATCTGAGCCACCAAA | |
pcDNA3.1-μNSΔ736-752 | Forward | GCCGCTCGAGTCTAGAGCCACCATG GACTACAAAGACGATGACGACAAGATGGCTGAATCAATTACTTTTG |
Reverse | AAACGGGCCCTCTAGATCAGTCGATGATTTTTGGAAACTC | |
pcDNA3.1-μNSΔ1-401 | Forward | GCCGCTCGAGTCTAGAGCCACCATGCCAACCACCTGGTATTCAAC |
Reverse | AAACGGGCCCTCTAGATCACTTGTCGTCATCGTCTTTGTAGTCGCCACGTAGCACATTATTCACGCC | |
pcDNA3.1-μNSΔ402-752 | Forward | GCCGCTCGAGTCTAGAGCCACCATG GACTACAAAGACGATGACGACAAGATGGCTGAATCAATTACTTTTG |
Reverse | AAACGGGCCCTCTAGATCATGTGGTCAGGGAATAGTGCAT |