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Table 1 Cell culture isolation of salmonid alphavirus from different tissues of experimentally challenged Atlantic salmon parr.

From: Pathogenesis of experimental salmonid alphavirus infection in vivo: an ultrastructural insight

Day post challenge

Log 10 TCID50/gram tissue

Tank

Fish

Heart

Kidney

Gill

Day 4

Tank 1

Fish 1

Negative

Negative

5.2

Fish 2

Negative

5.9

5.7

Tank 2

Fish 4

4.4

5.0

5.4

Fish 5

5.7

6.6

5.5

Day 11

Tank 1

Fish 1

6.6

7.1

6.4

Fish 2

Negative

5.3

5.7

Tank 2

Fish 4

Negative

6.1

5.3

Fish 5

Negative

6.6

6.1

  1. The 50% tissue culture infective dose (TCID50) of heart, gill and head kidney homogenate prepared from samples obtained from intraperitoneally challenged fish on 4 and 11 days post-challenge was estimated on 11 days post-inoculation on to CHSE-214 cells.