Table 2 Primers used for RT-PCR amplification.
From: Ultra-deep sequencing of VHSV isolates contributes to understanding the role of viral quasispecies
Name | Sequence | Amplicon (length in bp) | Overlapping region |
|---|---|---|---|
VHSV_Frag1I_nt18_+s* | GAGTTATGTTACARGGGACAGG | ||
VHSV_Frag1_nt2815_−s | CGATTGTAGYAGTCCTTCGC | Amplicon 1 (2797 bp) | Amplicon 1–2 = 274 bp |
VHSV_Frag2_nt2541_+s | GAGAAGATTGACTTCGGGAC | ||
VHSV_Frag2_nt6250_−s | CGTAGGTAGGAACCCTGTC | Amplicon 2 (3709 bp) | Amplicon 2–3 = 790 bp |
VHSV_Frag3_nt5460_+s | TACTGGAACTTGGCCTCACA | ||
VHSV_Frag3_nt8287_−s | TGTGTCCGCCAAATGGTGTA | Amplicon 3 (2827 bp) | Amplicon 3–4 = 431 bp |
VHSV_Frag4_nt7856_+s | GATGATTGTCTCCACCATGAA | ||
VHSV_Frag4I_nt11032_−s | TCTCCAAATGGAAAGAAGGACT | Amplicon 4 (3176 bp) |