Figure 4

Box plot of number of detected substitutions with intermediate allele frequencies (5–95%). Number of substitutions is represented as a count of substitution events recorded for polymorphic sites with intermediate allele frequencies (5–95%). Accordingly, substitution events resulting into alleles with frequencies below 5% were ignored. A The substitution counts for the individual gene across all four isolates for each of the six genes (four counts per gene). B Substitution counts for the individual isolate across all six genes for each of the four isolates (six counts per isolate).