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Figure 1 | Veterinary Research

Figure 1

From: Ultra-deep sequencing of VHSV isolates contributes to understanding the role of viral quasispecies

Figure 1

Generation of the four overlapping amplicons (1–4) to cover the entire viral genomes of each isolate. The VHSV isolates, are here abbreviated a DK-3592b; b DK-9895174; c 1p49; d 4p168. The individual RT-PCR reactions are shown in A; B re-amplified Amplicon 3 of sample b*; C amplification of the whole genome RT-PCR reaction; D pooled amplicon samples (1:1:1:1) for each of the VHSV samples used for library preparation.

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