Figure 5

Differential characteristics of CyHV-3 antigen expression observed by confocal microscopy in infected KF-1 cells. A CyHV-3 infected cells labelled with MAb 20F10 detecting virus capsid-associated antigen. A confluent region of the KF-1 cell monolayer is shown with both cytoplasmic and concentrated nuclear signals of CyHV-3, 7 dpi; B CyHV-3 infected cells labelled with MAb 10A9 detecting virus envelope glycoprotein. Upper micrograph the signals seen in the infected cells shown are associated with the periphery of the nucleus (pn) and plasma membrane (m); Lower micrograph the nucleus of same infected cell as the upper micrograph is shown stained by propidium iodide—the curved arrow highlights the viral signal around periphery of nucleus. Blue DAPI/propidium iodide stained nuclei (in overlay micrographs) apart from the lower micrograph in B; green FITC staining of virus. Thin arrows nuclear associated antigen; curved arrows membranous associated antigen; arrow heads cytoplasmic associated antigen.