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Figure 1 | Veterinary Research

Figure 1

From: Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence

Figure 1

SDS-PAGE and western blot analysis of sucrose gradient purified CyHV-3 using monoclonal antibodies 10A9 and 20F10. A Silver stained SDS-PAGE of sucrose gradient purified CyHV-3, American isolate H361. Lane 1 CyHV-3 proteins from 0.25 mg mL−1 purified CyHV-3; Lane 2 10–250 kDa molecular weight markers; Lane 3 size of protein marker of interest. B Western blot analysis of purified MAbs 10A9 and 20F10 screened against purified CyHV-3. Lane 1 MAb 10A9, Lane 2 MAb 20F10, Lane 3 relative size (MW) of proteins on membrane.

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