GFP tagged VLP production and characterization
. A GFP insertion design and the additional amino acid sequences. NGCP represents the N-terminal GFP-tagged CP, 220GCP represents the Ala220 GFP-tagged CP, CGCP indicates the C-terminal GFP-tagged CP and (GGGGS)3 represents the flexible linker. The black blocks represent CP and the rectangle frames indicate GFP. B Immuno-EM was performed to detect the GFP display status of CGV and CGV was immune-labeled with 5 nm gold particles using mouse anti-GFP or not as first antibody as indicated. The bar indicates 100 nm.