Prokaryotic expression of HV
. A His-tag insertion design and the additional amino acid sequences. NHCP represents the N-terminal His-tagged CP, IHCP represents the internal His-tagged CP, and CHCP indicates the C-terminal His-tagged CP. The black blocks represent CP. B Electron microscopy of RBS and different HV. Magnification is 30 k and the bar indicates 100 nm. C Production ratio of different VLP in the same expression volume. The ratios were generated by normalization of production of HV to that of RBS. All the data represented means for at least 10 batches of VLP preparation with 2 standard deviations indicated by the error bars.