Placental cytokine and
receptor transcript expression. Scatter-plot graphs of relative mRNA expression levels (as x-fold change) of A IFN-γ, IL-4, TNF-α, IL-10, IL-6, IL12p40 and TGF-β1 cytokines and (B)
Toll-like receptors (TLR) 2 and 4 in placenta after intravenous infection of ewes with 106 Nc-Spain7 N. caninum tachyzoites at day 40 (G1), day 90 (G2) and day 120 (G3) of gestation. Data are represented as individual points. Horizontal lines represent median values for each group. The horizontal discontinuous line set at 100 indicates uninfected animals baseline. (***), (**), (*) and (σ) symbols indicate P < 0.001, P < 0.01, P < 0.05 significant differences and a tendency to significance (P = 0.052), respectively.