Humoral and cellular immune responses in sera. Graphs representing A IgG and B IFN-γ levels in sera from ewes inoculated with 106 Nc-Spain7 tachyzoites at day 40 (G1), 90 (G2) and 120 (G3) of gestation, as well as control groups (G4 + G5). Each point represents the mean + SD at the different sampling times for each group. Data beyond 21 dpi is not represented since several animals did not maintain pregnancy and were therefore sacrificed. Sera levels of total IgG antibodies against N. caninum are expressed as a relative index percent (RIPC), according to the formula: RIPC = (OD405 sample–OD405 negative control)/(OD405 positive control–OD405 negative control) × 100. Concentrations of IFN-γ are expressed in pg/mL.