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Table 1 Extent and kinetics of modulated mRNA concentrations after stimulating pbMEC with live E. coli 1303 or S. uberis strain 0140J.

From: Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages

Gene

Pathogen

Time

1 h

3 h

24 h

TNF

E. coli 1303

5 ± 0

88 ± 1

34 ± 10

S. uberis 0140J

2 ± 1

11 ± 3

2 ± 1

IL6

E. coli 1303

3 ± 1

34 ± 0

7 ± 2

S. uberis 0140J

1 ± 0

3 ± 0

1 ± 0

CXCL8

E. coli 1303

6 ± 0

213 ± 9

36 ± 5

S. uberis 0140J

1 ± 0

17 ± 10

5 ± 2

CCL5

E. coli 1303

1 ± 0

14 ± 6

124 ± 85

S. uberis 0140J

1 ± 0

1 ± 0

1 ± 1

NOS2A

E. coli 1303

4 ± 1

137 ± 11

17 ± 6

S. uberis 0140J

1 ± 1

7 ± 1

1 ± 0

LAP

E. coli 1303

1 ± 0

5 ± 0

54 ± 8

S. uberis 0140J

1 ± 0

1 ± 0

2 ± 0

SAA3

E. coli 1303

1 ± 0

13 ± 1

72 ± 35

S. uberis 0140J

1 ± 0

2 ± 0

2 ± 1

CYP1A1

E. coli 1303

77 ± 12

445 ± 61

3 ± 1

S. uberis 0140J

79 ± 39

590 ± 122

5 ± 1

  1. pbMEC were co-cultured for 1 h with 107 CFU of the respective pathogen. Subsequently, pathogens were killed by adding 100 µg/mL gentamicin. mRNA was harvested either instantly (1 h time point) or after culturing for another 2 or 23 h (3 and 24 h time point, respectively). Values are means (±SEM) of fold change relative to the unstimulated control from two biological replica experiments, each assayed in duplicate. Numbers given in bold font indicate significant regulation
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Veterinary Research

ISSN: 1297-9716