|
Gene
|
Pathogen
|
Time
|
|---|
|
1 h
|
3 h
|
24 h
|
|---|
|
TNF
|
E. coli 1303
|
5 ± 0
|
88 ± 1
|
34 ± 10
|
|
S. uberis 0140J
|
2 ± 1
|
11 ± 3
|
2 ± 1
|
|
IL6
|
E. coli 1303
|
3 ± 1
|
34 ± 0
|
7 ± 2
|
|
S. uberis 0140J
|
1 ± 0
|
3 ± 0
|
1 ± 0
|
|
CXCL8
|
E. coli 1303
|
6 ± 0
|
213 ± 9
|
36 ± 5
|
|
S. uberis 0140J
|
1 ± 0
|
17 ± 10
|
5 ± 2
|
|
CCL5
|
E. coli 1303
|
1 ± 0
|
14 ± 6
|
124 ± 85
|
|
S. uberis 0140J
|
1 ± 0
|
1 ± 0
|
1 ± 1
|
|
NOS2A
|
E. coli 1303
|
4 ± 1
|
137 ± 11
|
17 ± 6
|
|
S. uberis 0140J
|
1 ± 1
|
7 ± 1
|
1 ± 0
|
|
LAP
|
E. coli 1303
|
1 ± 0
|
5 ± 0
|
54 ± 8
|
|
S. uberis 0140J
|
1 ± 0
|
1 ± 0
|
2 ± 0
|
|
SAA3
|
E. coli 1303
|
1 ± 0
|
13 ± 1
|
72 ± 35
|
|
S. uberis 0140J
|
1 ± 0
|
2 ± 0
|
2 ± 1
|
|
CYP1A1
|
E. coli 1303
|
77 ± 12
|
445 ± 61
|
3 ± 1
|
|
S. uberis 0140J
|
79 ± 39
|
590 ± 122
|
5 ± 1
|
- pbMEC were co-cultured for 1 h with 107 CFU of the respective pathogen. Subsequently, pathogens were killed by adding 100 µg/mL gentamicin. mRNA was harvested either instantly (1 h time point) or after culturing for another 2 or 23 h (3 and 24 h time point, respectively). Values are means (±SEM) of fold change relative to the unstimulated control from two biological replica experiments, each assayed in duplicate. Numbers given in bold font indicate significant regulation
