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Table 1 Extent and kinetics of modulated mRNA concentrations after stimulating pbMEC with live E. coli 1303 or S. uberis strain 0140J.

From: Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages

Gene Pathogen Time
1 h 3 h 24 h
TNF E. coli 1303 5 ± 0 88 ± 1 34 ± 10
S. uberis 0140J 2 ± 1 11 ± 3 2 ± 1
IL6 E. coli 1303 3 ± 1 34 ± 0 7 ± 2
S. uberis 0140J 1 ± 0 3 ± 0 1 ± 0
CXCL8 E. coli 1303 6 ± 0 213 ± 9 36 ± 5
S. uberis 0140J 1 ± 0 17 ± 10 5 ± 2
CCL5 E. coli 1303 1 ± 0 14 ± 6 124 ± 85
S. uberis 0140J 1 ± 0 1 ± 0 1 ± 1
NOS2A E. coli 1303 4 ± 1 137 ± 11 17 ± 6
S. uberis 0140J 1 ± 1 7 ± 1 1 ± 0
LAP E. coli 1303 1 ± 0 5 ± 0 54 ± 8
S. uberis 0140J 1 ± 0 1 ± 0 2 ± 0
SAA3 E. coli 1303 1 ± 0 13 ± 1 72 ± 35
S. uberis 0140J 1 ± 0 2 ± 0 2 ± 1
CYP1A1 E. coli 1303 77 ± 12 445 ± 61 3 ± 1
S. uberis 0140J 79 ± 39 590 ± 122 5 ± 1
  1. pbMEC were co-cultured for 1 h with 107 CFU of the respective pathogen. Subsequently, pathogens were killed by adding 100 µg/mL gentamicin. mRNA was harvested either instantly (1 h time point) or after culturing for another 2 or 23 h (3 and 24 h time point, respectively). Values are means (±SEM) of fold change relative to the unstimulated control from two biological replica experiments, each assayed in duplicate. Numbers given in bold font indicate significant regulation