Figure 4

LTA significantly activated immune gene expression and NF-κB in pbMEC, but not through TLR2 activation. A pbMEC were stimulated with 10 µg/mL of the respective LTA preparation for the time, as indicated (abscissa). TNF and CXCL8 mRNA concentrations were measured from duplicate assays, normalized against the CLIC1 reference and expressed as multiples of the concentration from unstimulated controls (*p < 0.05). B The same LTA preparations or their peroxide treated derivatives were used to stimulate pbMEC cultures having previously been transfected with the ELAM driven NF-κB reporter gene (left hand panel, stimulated with 10 µg/mL LTA) or HEK293 cells, having been co-transfected with that NF-κB reporter and the construct expressing the bovine TLR2 receptor. The different dose of the challenge substance is indicated. ^$ E. coli stimulation was with 30 µg/mL. These experiments are representative for three (left panel) or two (right panel) each assayed in triplicate.