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Figure 2 | Veterinary Research

Figure 2

From: Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages

Figure 2

Neither S. uberis mutants with defects in the formation of a hyaluronic acid capsule or in glycolipid biosynthesis nor an avirulent strain activated immune functions of pbMEC. A Induction of gene expression (ordinate) of TNF, CXCL8 and NOS2A after challenging with 30 µg/mL of heat-killed 0140J hasA mutant (ΔhasA) compared against two different preparations of wild-type 0140J (0140J wta/b) and E. coli 1303 for the time as indicated (abscissa). All preparations strongly induced the expression of CYP1A1. Values are from a single experiment representative for three, each assayed in duplicate. B Same experimental setting as before, but the challenge time was 3 h only. EF20, avirulent strain; 0140J::ISS1 P’, mutant defective for glycolipid biosynthesis. The experiment was assayed in triplicate. Asterisks indicate significance of the different mean values from the control (p < 0.05).

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