mutants with defects in the formation of a hyaluronic acid capsule or in glycolipid biosynthesis nor an avirulent strain activated immune functions of pbMEC.
A Induction of gene expression (ordinate) of TNF, CXCL8 and NOS2A after challenging with 30 µg/mL of heat-killed 0140J hasA mutant (ΔhasA) compared against two different preparations of wild-type 0140J (0140J wta/b) and E. coli
1303 for the time as indicated (abscissa). All preparations strongly induced the expression of CYP1A1. Values are from a single experiment representative for three, each assayed in duplicate. B Same experimental setting as before, but the challenge time was 3 h only. EF20, avirulent strain; 0140J::ISS1 P’, mutant defective for glycolipid biosynthesis. The experiment was assayed in triplicate. Asterisks indicate significance of the different mean values from the control (p < 0.05).