ELISPOT analyses to quantify PEDV specific IgA and IgG antibody secreting cell population in ileum, mesenteric lymph nodes, and spleen of PEDV infected sows. MNCs isolated from ileum (A, B, G, H), MLN (C, D, I, J), and spleen (E, F, K, L) were stimulated ex vivo with PEDV whole virus-derived antigen (B, D, F, H, J, L) or medium control (A, C, E, G, I, K) for 6 days. PEDV specific IgA (A–F) and IgG (G–L) antibody secreting cells (ASCs) were detected by ELISPOT. Each bar represents mean number of PEDV-specific ASCs per 5 × 105 MNCs from six sows. Lowercase alphabet indicates statistically significant difference between uninfected and PEDV-infected corresponding parity sow groups, and asterisk indicates statistical difference between the indicated PEDV-infected sow groups (“a” or *P < 0.05, “b” or **P < 0.01 and “c” or ***P < 0.001).