Table 1 Oligonucleotides used for construction of PrV vhs bearing point mutations in this study.
From: Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus
Oligonucleotides containing altered codons | |
|---|---|
D34-A(GAC-GCC) | (F) 5′-ACGCCCATCGCCATCGCCCTGTGGAACGTCATG-3′ (R) 5′-CATGACGTTCCACAGGGCGATGGCGATGGGCGT-3′ |
D81-A(GAC-GCC) | (F) 5′-CCCATCTTCGTCTCGGCCCGCGGCATCTTCGGG-3′ (R) 5′-CCCGAAGATGCCGCGGGCCGAGACGAAGATGGG-3′ |
K95-A(AAG-GCG) | (F) 5′-GTCGCGCACGGCGCCGCGGCCATCATGGCCGCG-3′ (R) 5′-CGCGGCCATGATGGCCGCGGCGCCGTGCGCGAC-3′ |
E150-A(GAG-GCG) | (F) 5′-GACGTGTCGGACATGGCGGCGGACGACGTCTGC-3′ (R) 5′-GCAGACGTCGTCCGCCGCCATGTCCGACACGTC-3′ |
D152-A(GAC-GCC) | (F) 5′-TCGGACATGGAGGCGGCCGACGTCTGCGCCAAC-3′ (R) 5′-GTTGGCGCAGACGTCGGCCGCCTCCATGTCCGA-3′ |
D169-A (ACG-GCG) | (F) 5′-GTCGCGCAGGTGCACGCGACCGACACGGACATG-3′ (R) 5′-CATGTCCGTGTCGGTCGCGTGCACCTGCGCGAC-3′ |
D171-A(GAC-GCC) | (F) 5′-CAGGTGCACACGACCGCCACGGACATGATCCTC-3′ (R) 5′-GAGGATCATGTCCGTGGCGGTCGTGTGCACCTG-3′ |
D173-A(GAC-GCC) | (F) 5′-CACACGACCGACACGGCCATGATCCTCACCGGG-3′ (R) 5′-CCCGGTGAGGATCATGGCCGTGTCGGTCGTGTG-3′ |
D219-A(GAC-GCC) | (F) 5′-GTGCGCTGCCACACGGCCCTGCACCGGGCGCCG-3′ (R) 5′-CGGCGCCCGGTGCAGGGCCGTGTGGCAGCGCAC-3′ |
172(delete ACG) | (F) 5′-GTGCACACGACGGAC-GACATGATCCTCACC-3′ (R) 5′-GGTGAGGATCATGTCGTCCGTCGTGTGCAC-3′ |
343(delete CCC) | (F) 5′-TTTTGGAAACACATA-CCGCCCCCGAACTAC-3′ (R) 5′-GTAGTTCGGGGGCGGTATGTGTTTCCAAAA-3′ |
345(delete CCC) | (F) 5′-AAACACATACCCCCG-CCGAACTACCAGGCC-3′ (R) 5′-GGCCTGGTAGTTCGGCGGGGGTATGTGTTT-3′ |
352(delete TCC) | (F) 5′-AACTACCAGGCCGTC-ATGGCGTACTGGGAC-3′ (R) 5′-GTCCCAGTACGCCATGACGGCCTGGTAGTT-3′ |
356(delete TGG) | (F) 5′-GTCCTCATGGCGTAC-GACGACTGTAACGCC-3′ (R) 5′-GGCGTTACAGTCGTCGTACGCCATGAGGAC-3′ |