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Table 1 Oligonucleotides used for construction of PrV vhs bearing point mutations in this study.

From: Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus

  Oligonucleotides containing altered codons
D34-A(GAC-GCC) (F) 5′-ACGCCCATCGCCATCGCCCTGTGGAACGTCATG-3′
(R) 5′-CATGACGTTCCACAGGGCGATGGCGATGGGCGT-3′
D81-A(GAC-GCC) (F) 5′-CCCATCTTCGTCTCGGCCCGCGGCATCTTCGGG-3′
(R) 5′-CCCGAAGATGCCGCGGGCCGAGACGAAGATGGG-3′
K95-A(AAG-GCG) (F) 5′-GTCGCGCACGGCGCCGCGGCCATCATGGCCGCG-3′
(R) 5′-CGCGGCCATGATGGCCGCGGCGCCGTGCGCGAC-3′
E150-A(GAG-GCG) (F) 5′-GACGTGTCGGACATGGCGGCGGACGACGTCTGC-3′
(R) 5′-GCAGACGTCGTCCGCCGCCATGTCCGACACGTC-3′
D152-A(GAC-GCC) (F) 5′-TCGGACATGGAGGCGGCCGACGTCTGCGCCAAC-3′
(R) 5′-GTTGGCGCAGACGTCGGCCGCCTCCATGTCCGA-3′
D169-A (ACG-GCG) (F) 5′-GTCGCGCAGGTGCACGCGACCGACACGGACATG-3′
(R) 5′-CATGTCCGTGTCGGTCGCGTGCACCTGCGCGAC-3′
D171-A(GAC-GCC) (F) 5′-CAGGTGCACACGACCGCCACGGACATGATCCTC-3′
(R) 5′-GAGGATCATGTCCGTGGCGGTCGTGTGCACCTG-3′
D173-A(GAC-GCC) (F) 5′-CACACGACCGACACGGCCATGATCCTCACCGGG-3′
(R) 5′-CCCGGTGAGGATCATGGCCGTGTCGGTCGTGTG-3′
D219-A(GAC-GCC) (F) 5′-GTGCGCTGCCACACGGCCCTGCACCGGGCGCCG-3′
(R) 5′-CGGCGCCCGGTGCAGGGCCGTGTGGCAGCGCAC-3′
172(delete ACG) (F) 5′-GTGCACACGACGGAC-GACATGATCCTCACC-3′
(R) 5′-GGTGAGGATCATGTCGTCCGTCGTGTGCAC-3′
343(delete CCC) (F) 5′-TTTTGGAAACACATA-CCGCCCCCGAACTAC-3′
(R) 5′-GTAGTTCGGGGGCGGTATGTGTTTCCAAAA-3′
345(delete CCC) (F) 5′-AAACACATACCCCCG-CCGAACTACCAGGCC-3′
(R) 5′-GGCCTGGTAGTTCGGCGGGGGTATGTGTTT-3′
352(delete TCC) (F) 5′-AACTACCAGGCCGTC-ATGGCGTACTGGGAC-3′
(R) 5′-GTCCCAGTACGCCATGACGGCCTGGTAGTT-3′
356(delete TGG) (F) 5′-GTCCTCATGGCGTAC-GACGACTGTAACGCC-3′
(R) 5′-GGCGTTACAGTCGTCGTACGCCATGAGGAC-3′