Figure 3
From: Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus
An in vitro assay of RNase activity mediated by PrV vhs using various substrates. (A) Different types of RNA, including single stranded RNA without cap (ssRNA), with cap and polyA tail (mRNA) and cellular total rRNA, were incubated with assay buffer (mock), recombinant vhs and the appropriate tag proteins, either NUS or Thioredoxin (THX) (negative control), for various times (as indicated above the gel). The degradation pattern was resolved by agarose electrophoresis. In addition to RNA, various DNA substrates (B) and a DNA-RNA hybrid (C) were also tested.