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Table 1 Summary of the trials and sets to evaluate OsHV-1 and V. aestuarianus susceptibility.

From: Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures

Trial 1 2 3 3 3 3 3 4 4
Set    1 2 3 4 5a 1b 2c
Pathogen OsHV-1 Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus Vibrio aestuarianus
Infection protocol Cohabitation Injection Cohabitation Cohabitation Cohabitation Cohabitation Cohabitation Cohabitation Cohabitation
Number of exposure 1st infection 1st infection 1st infection 1st infection 1st infection 1st infection 1st infection 2nd infection 3rd infection
Date of challenge Apr 2013 Jan 2013 Feb 2013 Mar 2013 Mar 2013 May 2014 Nov 2014 May 2014 Nov 2014
Age (months) 13 10 11 12 13 26 32 26 32
Individual weight (g) 22 22 22 22 22 120 100 100 170
Stage Juvenile Juvenile Juvenile Juvenile Juvenile Adult Adult Adult Adult
Number of tanksf 12 48 3 3 8 2 2 1 1
Tank volume (L) 5 5 150 150 10 150 150 150 150
Lines tank−1 1 1 4 4 1 4 4 4 3d
Oysters line-1 tank−1 10 10 25 25 15–20 24–32 28–33 1–88e 1–30e
OsHV-1 detection in moribunds Yes No No No No No No No No
V. aestuarianus detection in moribunds No Yes Yes Yes Yes Yes Yes Yes Yes
  1. For all trials, two unselected and control lines (AC and BC) and two lines selected for their higher resistance to OsHV-1 (AS and BS) were evaluated under controlled conditions
  2. aOysters used were survivors from field testing
  3. bOysters used were survivors from sets 1 to 3 of trial 3
  4. cOysters used were survivors from set 1 of trial 4
  5. dOne of the control line had 100% mortality before the trial
  6. eSelected lines had at least 28 and 12 oysters in sets 4 and 5, respectively, whereas the control lines had less than 5 oysters
  7. fThe number indicated is without the control tanks