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Table 2 Data of experimentally infected hamsters.

From: Natural transmission of Leishmania infantum through experimentally infected Phlebotomus perniciosus highlights the virulence of Leishmania parasites circulating in the human visceral leishmaniasis outbreak in Madrid, Spain

Group Transmission route Leishmania strain or isolate No. of exposed sand fliesa No. of infective bites (%)b Culture Parasite burdenc
Spleen Liver Spleen Liver
H1 Control X X X
X X
X X
X X
H2 Bite JPCM5 55 14/55 (25.5%) + 95
55 13/55 (23.6%) 4 × 102
55 16/55 (29.1%)
55 14/55 (25.5%)
H3 Bite JPCM5 49 25/49 (51.0%) +
49 21/49 (42.9%)
49 14/49 (28.6%)
49 14/49 (28.6%) + 3.1 × 103
H4 IP JPCM5 X X + 2.6 × 102
X X
X X
X X
H5 Bite POL2FL6 25 1 Attemp
25 1 Attemp
25 >5 Attemps 3.3 × 105 2.9 × 104
25 3 Attemps + + 8.8 × 1012 2.0 × 1010
H6 Bite POL2FL6 25 1/25 (4.0%) + 6.5 × 105 6.7 × 108
25 1/25 (4.0%) 1.3 × 104
17 1/17 (5.9%)
H7 IP POL2FL6 X X + + 2.3 × 1013 2.1 × 1012
X X + + 3.9 × 1011 5.3 × 1010
X X + + 2.4 × 1013 1.9 × 1012
X X + + 6.4 × 1012 5.9 × 1011
H8 IP BOS1FL1 X X + + 3.2 × 1012 9.3 × 1010
X X + + 6.3 × 1013 5.9 × 1012
  1. aThe number of sand flies used for Leishmania challenge depended on the amount of female flies that survived oviposition. Survival flies were divided into cages where animals were placed for challenge
  2. bThe number of infective bites that each animal received is calculated as the number of flies that took a blood meal and was shown to be infected by Leishmania, as checked under the microscope, divided by the number of exposed flies ×100
  3. cParasite burden is expressed as the number of parasites per gram of tissue