Relative gene expression analysis in gastrohepatic lymph node of ASFV-infected pigs (experiment 2). Statistical analysis (using T-Student test: *p < 0.05; **p < 0.01 and ***p < 0.001) comparing the RNA expression levels found for key regulatory molecules in gastrohepatic lymph nodes of animals infected with E75CV1 (attenuated) or E75 (virulent) homologous ASFV strains, relative to non-infected animals (control group). Values corresponding to three pigs per group and times are represented: 1 day (A), 7 days (B) and 31 days (C) post-infection. The colour grade reflects the fold-change differences found (the higher the transcription up or down regulation, the darker the colour): the green gradient represents transcription down-regulation (from −1.3 to −fivefold changes) and the red gradient represents transcription up-regulation (from 1.3–threefold changes). No colour (white) indicates no changes in the corresponding transcript level. No survivors from the E75 ASFV inoculated pigs were available at day 31 pi.