Veterinary Research

Table 1 Number of fish positive for ISAV in gills, hind gut, skin/pectoral fin, eye, and skin/mucus.

From: Infectious salmon anaemia virus (ISAV) mucosal infection in Atlantic salmon

Day	6 h	1	2	3	4	5	6	7	8	10	12	14	19	23
Gills	4	4	4	4	4	4	4	4	4	4	4	4	4	4	qPCR	Low
	0/0	0/0	0/4	0/4	2/4	3/1	4/1	4/1	2/2	3/2	4/3	3/na	4/na	1/na	IHC/IFAT	Low
	4	4	4	4	4	4	4	4	4	4	4	4	4	4	qPCR	High
	0/0	0/0	0/0	0/1	0/3	1/0	3/2	2/2	2/3	4/4	4/4	4/na	4/na	4/na	IHC/IFAT	High
Hind gut	0	2	3	3^a	4	4	4	4	4	4	4	4	4	4	qPCR	Low
	0	0	0	0	1	2	0	3	1	4	3	1	2	0	IHC	Low
	0	1	0	4	4	4	4	4	4	4	4	4	4	3^b	qPCR	High
	0	0	0	2	0	0	1	1	1	3	4	3	3	3	IHC	High
Skin and/or fin	4	4	4	2^c	4	4	4	4	4	4	4	4	4	na	qPCR	Low
	0	0	0	0	3	4	4	4	0	4	4	4	4	2	IHC	Low
	3	3	4	4	4	4	3	4	4	4	4	4	4	na	qPCR	High
	0	0	0	1	3	4	4	4	0	3	4	4	4	4	IHC	High
Eye (n = 2)	0	0	0	0	0	0	2	0	1	1	2	2	0	0	IHC	Low
Eye (n = 2)	0	0	0	0	0	0	1	1	0	2	2	2	0	0	IHC	High
Mucus	4	4	4	4	4	4	4	4	4	4	4	4	4	na	qPCR	Low
Mucus	4	4	4	4	4	4	4	4	4	4	4	4	4	na	qPCR	High

Number of fish positive for ISAV in gills, hind gut, skin/pectoral fin (fin only for RT-qPCR), eye, and skin/mucus swabs with RT-qPCR or IHC/IFAT in fish infected with low and highly virulent ISAV
na not applicable
^aThe “negative” sample was much smaller than the others and produced an ELF Cp 9 cycles higher than expected
^bOnly 3 fish tested
^cOnly 2 fish tested

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ISSN: 1297-9716

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