Table 1 List of the primers used in the qPCR assay
From: F4+ ETEC infection and oral immunization with F4 fimbriae elicits an IL-17-dominated immune response
Gene | Sequence (5′→3′) | Size (bp) | Ta (°C) | Reference |
|---|---|---|---|---|
IL-17A | F:ACTCCAAACGCTTCACCTCAC | 234 | 58 | NM_001005729.1 |
R:AGCCCACTGTCACCATCACTT | ||||
IL-17B-like | F:CTGGCCAAGAGGAAGTGTGAG | 92 | 60 | XM_003124086.1 |
R:GGGTCGTGGTTGATGCTGTAG | ||||
IL-17F | F:GAGGCAGCAGCTCGGAAAAT | 173 | 60 | NC_010449.4 |
R:TCCCGGGTGATGTTGTAATCC | ||||
IL-21 | F:GGCACAGTGGCCCATAAATC | 124 | 60 | [30] |
R:GCAGCAATTCAGGGTCCAAG | ||||
IL-22 | F:TTGACCAGTCCAACTTCCAGCAGC | 143 | 60 | XM_001926156.1 |
R:GCAGCGCTCTCTCATATTGACTCC | ||||
IL-23p19 | F:CCAAGAGAAGAGGGAGATGATGA | 107 | 57 | NM_001130236.1 |
R:TGCAAGCAGGACTGACTGTTGT | ||||
RORγt | F:TTCAGTACGTGGTGGAGTTC | 141 | 60 | [30] |
R:TGTGGTTGTCAGCGTTGTAG | ||||
IL-10 | F : CCATGCCCAGCTCAGCACTG | 295 | 60 | [65] |
R:CCCATCACTCTCTGCCTTCGG | ||||
IL-13 | F:GTCATTGCTCTCACCTGCTT | 308 | 58 | [66] |
R:TTGGTGTCTCGGATGTGCTT | ||||
GATA-3 | F:GCTCTACCACAAAATGAACGGAC | 110 | 58 | NM_001044567.1 |
R:TCGTTGTGGTTTGACAGTTTGC | ||||
IL-12p40 | F:GGTTTCAGACCCGACGAACTCT | 112 | 60 | [67] |
R:CATATGGCCACAATGGGAGATG | ||||
IFN-γ | F:GAGCCAAATTGTCTCCTTCTACT | 262 | 60 | [68] |
R:CTGACTTCTCTTCCGCTTTCT | ||||
T-bet | F:TCAATCCTACTGCCCACTAC | 151 | 60 | [69] |
R:TTAGGAGACTCTGGGTGAAC | ||||
Foxp3 | F:TGCCATTCGCCACAACTT | 179 | 60 | NM_001128438.1 |
R:CCTGTCCATCCTTCTTTCCTT | ||||
AID | F:AGAAGTTTCAAAGCCTGGGAG | 92 | 57 | XM_003126511.1 |
R:TCAACCTCATACAGGGGCAAA | ||||
β-Actin | F:TCATCACCATCGGCAACG | 133 | 60 | [70] |
R:TTCCTGATGTCCACGTCGC | ||||
GAPDH | F:GGGCATGAACCATGAGAAGT | 230 | 60 | [65] |
R:AAGCAGGGATGATGTTCTGG | ||||
RPL19 | F:AACTCCCGTCAGCAGATCC | 147 | 60 | [69] |
R:AGTACCCTTCCGCTTACCG |