The mRNA expression profile in PBMCs triggered by F4
ETEC infection. Piglets were infected with F4+ ETEC on day 0 (D0) and day 1 (D1). PBMCs were isolated from piglets on D0 until D4 after infection. The mRNA expression in the PBMCs of F4+ ETEC infected and control piglets was analyzed by qPCR. The mRNA expression level was normalized to the reference genes and then to the control group for every separate day. Then, the data were plotted relative to D0. Data are presented as the mean ± SEM (n = 3 per group). * p < 0.05, ** p < 0.01.