Viral RNA loads follow-up in sera and feathers after infection with lineage 2 WNV strains. Mean viral loads in sera A and feathers B were estimated by NS2a-specific rRT-PCR in day-old SPF chicks experimentally inoculated with either Hun2004 (black bars), Aus2008 (hatched bars) or Gr2011 (white bars). Error bars represent the standard error of the mean. Above the bars, numbers of positives/examined samples are indicated. *P < 0.05 for Hun2004 and Aus2008 versus Gr2011 at 2 dpi. #
P < 0.05 for Hun2004 versus Aus2008 versus Gr2011 at 5 dpi. $
P < 0.05 for Aus2008 versus Gr2011 at 2 dpi. &
P < 0.05 for Aus2008 versus Hun2004 and Gr2011 at 14 dpi. NS2a-specific rRT-PCR detection limit is 101.67 RNA copies/µL.